Analysis Note

Positive ControlHEK293 cells

Application

Immunoblotting (0.25 g/ml)Immunofluorescence (Please see comments)Immunoprecipitation (1 g/ml)

General description

Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³² in HEK293 cells.

This PhosphoDetect™ Anti-PDH-E1α (pSer²³²) Rabbit pAb is validated for use in Immunoblotting, IF, IP for the detection of PDH-E1α (pSer²³²).

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser²³².

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³² phosphorylation site of human PDH-E1α

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.Seifert, F., et al. 2007. Biochemistry 21, 6277.Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

This antibody has been known to work with immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Packaging

50 µg in Plastic ampoule

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)